pRK5myc Rac1 L61
(Plasmid #15903)

• Depositing Lab: Alan Hall
• Publication: Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44.

Backbone

• Vector backbone: pRK5-myc
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rac1
• Alt name: Isoform 1 of Rac
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Mutation: Q61L
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constitutively activated Rac.