PB-CAG::Nucbow
(Plasmid
#158996)
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PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCX
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Backbone manufacturerGenscript inc
- Backbone size w/o insert (bp) 2530
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Vector typeMammalian Expression
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Selectable markersH2B-EBFP2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNucbow
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SpeciesSynthetic
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Insert Size (bp)7959
- Promoter CAG
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Tag
/ Fusion Protein
- All FPs are fused to H2B (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-CAG::Nucbow was a gift from Jean Livet (Addgene plasmid # 158996 ; http://n2t.net/addgene:158996 ; RRID:Addgene_158996) -
For your References section:
Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016. 10.1016/j.neuron.2013.12.016 PubMed 24507188
