Tol2-CAG::Mitbow
(Plasmid #158994)

• Purpose: Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
• Depositing Lab: Jean Livet
• Publication: Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.

Backbone

• Vector backbone: pCX
• Backbone manufacturer: Genscript inc
• Backbone size w/o insert (bp): 2006
• Vector type: Mammalian Expression
• Selectable markers: H2B-EBFP2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mitbow
• Species: Synthetic
• Insert Size (bp): 7410
• Promoter: CAG
• Tag / Fusion Protein:
  • The default expressed EBFP2 is fused to H2B; other FPs have a mitochondrial N tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry