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Addgene

Tol2-CAG::Palmbow
(Plasmid #158993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCX
  • Backbone manufacturer
    Genscript inc
  • Backbone size w/o insert (bp) 2006
  • Vector type
    Mammalian Expression
  • Selectable markers
    H2B-EBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Palmbow
  • Species
    Synthetic
  • Insert Size (bp)
    7004
  • Promoter CAG
  • Tag / Fusion Protein
    • The default expressed EBFP2 is fused to H2B; other FPs have a palmitoyl N tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-CAG::Palmbow was a gift from Jean Livet (Addgene plasmid # 158993 ; http://n2t.net/addgene:158993 ; RRID:Addgene_158993)

  • For your References section:

    Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016. 10.1016/j.neuron.2013.12.016 PubMed 24507188
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