GAL4-DrBphP
(Plasmid #158986)

• Purpose: Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells
• Depositing Lab: Liangcai Gu
• Publication: Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.1021/acssynbio.0c00397.

Backbone

• Vector backbone: pBobi
• Backbone size w/o insert (bp): 8877
• Total vector size (bp): 10905
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GAL4-DrBphP
• Species: Deinococcus radiodurans
• Insert Size (bp): 2028
• GenBank ID: NC_001264.1
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc tag (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGC TCG TTT AGT GAA CCG TCA GAT CG
• 3′ sequencing primer: AAT TCC TCG GTA CCA CCG GTT C

Resource Information

• Supplemental Documents:
  • GAL4-DrBphP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry