pAAV_2pabPAC
(Plasmid
#158969)
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Purpose2P-activatable bPAC for AAV
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerCell Biolabs Inc
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 7100
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert name2pabPAC
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Alt namemTFP1(d11)_bPAC_P2Av3_mTFP1
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SpeciesBeggiatoa sp and Clavularia sp.
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Insert Size (bp)2500
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGR-PA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_2pabPAC was a gift from Michiyuki Matsuda (Addgene plasmid # 158969 ; http://n2t.net/addgene:158969 ; RRID:Addgene_158969) -
For your References section:
Single-Cell Activation of the cAMP-Signaling Pathway in 3D Tissues with FRET-Assisted Two-Photon Activation of bPAC. Kinjo T, Watabe T, Kobachi K, Terai K, Matsuda M. ACS Chem Biol. 2020 Oct 19. doi: 10.1021/acschembio.0c00333. 10.1021/acschembio.0c00333 PubMed 33074647