pCAGGS_2pabPAC
(Plasmid
#158968)
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Purpose2P-activatable bPAC for transient expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 6500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name2pabPAC
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Alt namemTFP1(d11)_bPAC
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SpeciesS. cerevisiae (budding yeast), Synthetic; Beggiatoa sp and Clavularia sp.
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Insert Size (bp)2500
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAGGS-5
- 3′ sequencing primer rbglobpA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS_2pabPAC was a gift from Michiyuki Matsuda (Addgene plasmid # 158968 ; http://n2t.net/addgene:158968 ; RRID:Addgene_158968) -
For your References section:
Single-Cell Activation of the cAMP-Signaling Pathway in 3D Tissues with FRET-Assisted Two-Photon Activation of bPAC. Kinjo T, Watabe T, Kobachi K, Terai K, Matsuda M. ACS Chem Biol. 2020 Oct 19. doi: 10.1021/acschembio.0c00333. 10.1021/acschembio.0c00333 PubMed 33074647
