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Addgene

VanR - F165V
(Plasmid #158965)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158965 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416
  • Backbone size w/o insert (bp) 5650
  • Total vector size (bp) 6385
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VanR - F165V
  • Insert Size (bp)
    735
  • Mutation
    Wild type VanR from Caulobacter crescentus with mutation F165V
  • Promoter TEF1

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATCTGTCAUAAAACAATGGACATGCC
  • 3′ sequencing primer CACGCGAUTATCAGTCTGCTCTTATA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VanR - F165V was a gift from Michael Jensen (Addgene plasmid # 158965 ; http://n2t.net/addgene:158965 ; RRID:Addgene_158965)

  • For your References section:

    Directed evolution of VanR biosensor specificity in yeast. D'Ambrosio, V., Pramanik, S., Goroncy, K., Jakociunas, T., Schonauer, D., Davari, H. D., Schwaneberg, U., Keasling, J.D., Jensen, M.K.. Biotechnology Notes, Volume 1, 2020, Pages 9-15 10.1016/j.biotno.2020.01.002
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