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Addgene

pBS U6a gRNA
(Plasmid #158961)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158961 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Total vector size (bp) 3361
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    zf U6a promoter
  • Species
    D. rerio (zebrafish), Synthetic
  • Insert Size (bp)
    355

Gene/Insert 2

  • Gene/Insert name
    gRNA backbone
  • Species
    Synthetic
  • Insert Size (bp)
    76

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS U6a gRNA was a gift from Michael Brand (Addgene plasmid # 158961 ; http://n2t.net/addgene:158961 ; RRID:Addgene_158961)

  • For your References section:

    Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish. Hans S, Zoller D, Hammer J, Stucke J, Spiess S, Kesavan G, Kroehne V, Eguiguren JS, Ezhkova D, Petzold A, Dahl A, Brand M. Nat Commun. 2021 Feb 18;12(1):1125. doi: 10.1038/s41467-021-21427-6. 10.1038/s41467-021-21427-6 PubMed 33602923
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