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pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)
(Plasmid #158952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcdna3.1
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6520
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAD51
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1020
  • Mutation
    changed phenylalanine 129 to alanine
  • GenBank ID
    49168601
  • Entrez Gene
    RAD51 (a.k.a. BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2X FLAG 2X STREP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bamh1 (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGHR reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A) was a gift from Michele Pagano (Addgene plasmid # 158952 ; http://n2t.net/addgene:158952 ; RRID:Addgene_158952)

  • For your References section:

    The F-Box Domain-Dependent Activity of EMI1 Regulates PARPi Sensitivity in Triple-Negative Breast Cancers. Marzio A, Puccini J, Kwon Y, Maverakis NK, Arbini A, Sung P, Bar-Sagi D, Pagano M. Mol Cell. 2019 Jan 17;73(2):224-237.e6. doi: 10.1016/j.molcel.2018.11.003. Epub 2018 Dec 13. 10.1016/j.molcel.2018.11.003 PubMed 30554948
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