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Addgene

pRK7-Rheb
(Plasmid #15888)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15888 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRK7
  • Backbone size w/o insert (bp) 4708
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rheb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    550
  • Entrez Gene
    RHEB (a.k.a. RHEB2)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK7-Rheb was a gift from John Blenis (Addgene plasmid # 15888 ; http://n2t.net/addgene:15888 ; RRID:Addgene_15888)
  • For your References section:

    Tuberous sclerosis complex gene products, Tuberin and Hamartin, control mTOR signaling by acting as a GTPase-activating protein complex toward Rheb. Tee AR, Manning BD, Roux PP, Cantley LC, Blenis J. Curr Biol. 2003 Aug 5. 13(15):1259-68. 10.1016/S0960-9822(03)00506-2 PubMed 12906785