pVasa-Cre
(Plasmid #15885)

• Depositing Lab: Diego Castrillon
• Publication: Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse vasa promoter
• Alt name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Species: M. musculus (mouse)
• Insert Size (bp): 5600
• GenBank ID: NC_000079
• Entrez Gene: Ddx4 (a.k.a. AV206478, Mv, Mvh, VASA)
• Tag / Fusion Protein:
  • Cre (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: TGTGCCACCATGCCTGGCCCAGTTTC
• 3′ sequencing primer: TCTCCGCTCCAGGCTCCCCGGGCTCCT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence the lab cloned is somewhat different from the NCBI sequence. This could be explained by different strains, etc., since they cloned the promoter from the FVB strain whereas the NCBI genome sequence is C57Bl6. Since the plasmid has been shown to be functional in transgenic mice, the depositing lab does not believe this represents a problem.