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PurposepRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSF-Duet1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasPhi-2
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Alt nameCas12j2
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Tag
/ Fusion Protein
- 2xSV40NLS-6xHis (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPP537 was a gift from Jennifer Doudna (Addgene plasmid # 158803 ; http://n2t.net/addgene:158803 ; RRID:Addgene_158803) -
For your References section:
CRISPR-CasPhi from huge phages is a hypercompact genome editor. Pausch P, Al-Shayeb B, Bisom-Rapp E, Tsuchida CA, Li Z, Cress BF, Knott GJ, Jacobsen SE, Banfield JF, Doudna JA. Science. 2020 Jul 17;369(6501):333-337. doi: 10.1126/science.abb1400. 10.1126/science.abb1400 PubMed 32675376