pAS18
(Plasmid
#158787)
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PurposeRibosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSM
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Backbone manufacturerC.I. Bargmann and S. MaCarroll
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePtag-168::GCaMP6m::rpl-1a
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAS18 was a gift from Jennifer Garrison & Zachary Knight (Addgene plasmid # 158787 ; http://n2t.net/addgene:158787 ; RRID:Addgene_158787) -
For your References section:
Soma-Targeted Imaging of Neural Circuits by Ribosome Tethering. Chen Y, Jang H, Spratt PWE, Kosar S, Taylor DE, Essner RA, Bai L, Leib DE, Kuo TW, Lin YC, Patel M, Subkhangulova A, Kato S, Feinberg EH, Bender KJ, Knight ZA, Garrison JL. Neuron. 2020 Jun 5. pii: S0896-6273(20)30351-2. doi: 10.1016/j.neuron.2020.05.005. 10.1016/j.neuron.2020.05.005 PubMed 32574560