pyc111f
(Plasmid #158780)

• Purpose: ribosome-tagged, Cre-dependent GCaMP for soma localization
• Depositing Labs: Jennifer Garrison, Zachary Knight
• Publication: Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)30351-2. doi: 10.1016/j.neuron.2020.05.005.

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Backbone

• Vector backbone: Addgene #50457
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GCaMP6f-RPL10a
• Species: M. musculus (mouse)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pyc111f_hSyn-DIO-GCaMP6f-RPL10a-WPRE-HGH.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry