CMV-Tol2 transposase
(Plasmid #158774)

• Purpose: Expression of Tol2 transposase
• Depositing Lab: Hugo Snippert
• Publication: Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.

Backbone

• Vector backbone: pDB739
• Backbone size w/o insert (bp): 2927
• Total vector size (bp): 6108
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tol2 tranposase
• Species: Synthetic
• Insert Size (bp): 1950
• Mutation: Tol2 codon usage frequency well suitable for murine or human cells

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid obtained from Stefan Schulte-Merker.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.

Rapid BAC selection for tol2-mediated transgenesis in zebrafish. Bussman J, Schulte-Merker S. Development. 2011;138(19):4327-4332. doi:10.1242/dev.068080