pEF NS34a-msGFP-'Q-mito
(Plasmid
#158764)
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PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158764 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF-GFP (#11154)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNS3/4a protease and msGFP
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SpeciesHepatitis C virus
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MutationFlexible linkers between the protease and msGFP and msGFP and the cleavage site, EDVVCC/QMSY cleavage site
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In-fusion cloning was used for constuction of this plasmid
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF NS34a-msGFP-'Q-mito was a gift from Edwin Chapman (Addgene plasmid # 158764 ; http://n2t.net/addgene:158764 ; RRID:Addgene_158764) -
For your References section:
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. Vevea JD, Chapman ER. eLife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469. 10.7554/eLife.56469 PubMed 32515733
