EGFP-UTRN-mini
(Plasmid #158749)

• Purpose: Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells
• Depositing Lab: Minhajuddin Sirajuddin
• Publication: Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.15252/embj.2019104006.

Backbone

• Vector backbone: pCS2+
• Backbone manufacturer: Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
• Backbone size w/o insert (bp): 4823
• Total vector size (bp): 5123
• Modifications to backbone: Inserted UTRN-mini gene after eGFP in the Addgene plasmid # 26737 by Gibson cloning.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UTRN-mini
• Alt name: Utrophin-mini
• Alt name: Shorter actin binding Utrophin variant (amino acid 35-136)
• Insert Size (bp): 309
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • EGFP-UTRN-mini.gb
• A portion of this plasmid was derived from a plasmid made by: Received the plasmid # 26737 from Addgene and introduced UTRN-mini gene after eGFP by Gibson cloning.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry