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Addgene

pHCKAn-phoAp-GRFT
(Plasmid #158747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSMART HCKan
  • Backbone manufacturer
    Lucigen
  • Backbone size w/o insert (bp) 1788
  • Total vector size (bp) 2398
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phoAp Griffithsin
  • Alt name
    phoA GRFT
  • Species
    Synthetic
  • Insert Size (bp)
    610
  • Promoter phoA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer 5'-CAG TCC AGT TAC GCT GGA GTC
  • 3′ sequencing primer 5'-GGT CAG GTA TGA TTT AA A TGG TCA GT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCKAn-phoAp-GRFT was a gift from Michael Lynch (Addgene plasmid # 158747 ; http://n2t.net/addgene:158747 ; RRID:Addgene_158747)

  • For your References section:

    Low-Cost, Large-Scale Production of the Anti-viral Lectin Griffithsin. Decker JS, Menacho-Melgar R, Lynch MD. Front Bioeng Biotechnol. 2020 Aug 21;8:1020. doi: 10.3389/fbioe.2020.01020. eCollection 2020. 10.3389/fbioe.2020.01020 PubMed 32974328
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