pHCKAn-yibDp-GRFT
(Plasmid
#158745)
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Purposelow phosphate yibDp induction of Griffithsin
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158745 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSMART HCKan
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Backbone manufacturerLucigen
- Backbone size w/o insert (bp) 1788
- Total vector size (bp) 2443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameyibDp Griffithsin
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Alt nameyibD GRFT
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SpeciesSynthetic
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Insert Size (bp)655
- Promoter yibD
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer 5'-CAG TCC AGT TAC GCT GGA GTC
- 3′ sequencing primer 5'-GGT CAG GTA TGA TTT AA A TGG TCA GT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCKAn-yibDp-GRFT was a gift from Michael Lynch (Addgene plasmid # 158745 ; http://n2t.net/addgene:158745 ; RRID:Addgene_158745) -
For your References section:
Low-Cost, Large-Scale Production of the Anti-viral Lectin Griffithsin. Decker JS, Menacho-Melgar R, Lynch MD. Front Bioeng Biotechnol. 2020 Aug 21;8:1020. doi: 10.3389/fbioe.2020.01020. eCollection 2020. 10.3389/fbioe.2020.01020 PubMed 32974328
