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pPICZ-His6-K2P4.1A-mCherry-StreptagII
(Plasmid #158743)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158743 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZ
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 4832
  • Vector type
    Yeast Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    K2P4.1-A
  • Alt name
    TRAAK
  • Alt name
    KCNK4
  • Species
    H. sapiens (human)
  • Mutation
    Residues 1-264 with N-linked glycosylation sites mutated from Asn to Qln
  • Entrez Gene
    KCNK4 (a.k.a. FHEIG, K2p4.1, TRAAK, TRAAK1)
  • Promoter AOX1
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • mCherry with Streptag II (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GACTGGTTCCAATTGACAAGC
  • 3′ sequencing primer GCAAATGGCATTCTGACATCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPICZ-His6-K2P4.1A-mCherry-StreptagII was a gift from Arthur Laganowsky (Addgene plasmid # 158743 ; http://n2t.net/addgene:158743 ; RRID:Addgene_158743)

  • For your References section:

    Selective regulation of human TRAAK channels by biologically active phospholipids. Schrecke S, Zhu Y, McCabe JW, Bartz M, Packianathan C, Zhao M, Zhou M, Russell D, Laganowsky A. Nat Chem Biol. 2021 Jan;17(1):89-95. doi: 10.1038/s41589-020-00659-5. Epub 2020 Sep 28. 10.1038/s41589-020-00659-5 PubMed 32989299
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