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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16
(Plasmid #158742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCD5
  • Backbone manufacturer
    -
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 6690
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Neuraminidase A/NL/354/16 H3N2
  • Species
    Human
  • Insert Size (bp)
    1227
  • Mutation
    AA61-469
  • GenBank ID
    EPI1436648
  • Promoter CMV
  • Tag / Fusion Protein
    • TwinStrep_sfGFP_tetrabrachion (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG C
  • 3′ sequencing primer TTC AGG GGG AGG TGT GGG AG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16 was a gift from Robert de Vries (Addgene plasmid # 158742 ; http://n2t.net/addgene:158742 ; RRID:Addgene_158742)

  • For your References section:

    Drivers of recombinant soluble influenza A virus hemagglutinin and neuraminidase expression in mammalian cells. van der Woude R, Turner HL, Tomris I, Bouwman KM, Ward AB, de Vries RP. Protein Sci. 2020 Jul 25. doi: 10.1002/pro.3918. 10.1002/pro.3918 PubMed 32710576
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