pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS
(Plasmid #158739)

• Purpose: Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP
• Depositing Lab: Robert de Vries
• Publication: van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/pro.3918.

Backbone

• Vector backbone: pCD5
• Backbone manufacturer: -
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6935
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MC1061
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Hemagglutinin A/TX/50/12 H3
• Insert Size (bp): 1525
• Mutation: G186V S219F
• GenBank ID: AGL06219.1
• Promoter: CMV
• Tag / Fusion Protein:
  • GCN4-TEV-sfGFP-TwinStrep (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: GGC TAA CTA GAG AAC CCA CTG C
• 3′ sequencing primer: TTC AGG GGG AGG TGT GGG AG

Resource Information

• Supplemental Documents:
  • pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry