scFLARE1
(Plasmid
#158738)
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PurposeMammalian expression of scFLARE1 component
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 3802
- Total vector size (bp) 7705
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namescFLARE1
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SpeciesSynthetic
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Insert Size (bp)3900
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MutationTruncated form of TEV protease (220-242 amino acids deleted, low affinity). S219V mutation improves stability, S153N mutation improves catalytic activity. Calmodulin from GCaMP5
- Promoter CMV
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Tag
/ Fusion Protein
- Flag, V5
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-f
- 3′ sequencing primer PolyA-r (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
scFLARE1 was a gift from Alice Ting (Addgene plasmid # 158738 ; http://n2t.net/addgene:158738 ; RRID:Addgene_158738) -
For your References section:
Transcriptional readout of neuronal activity via an engineered Ca(2+)-activated protease. Sanchez MI, Nguyen QA, Wang W, Soltesz I, Ting AY. Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 10.1073/pnas.2006521117. 10.1073/pnas.2006521117 PubMed 33323488