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Addgene

pK7WG2-mitoTAL1-orf125
(Plasmid #158736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7WG2
  • Vector type
    TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitoTALEN left and right to target orf125 (orf138)
  • Species
    Synthetic

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Tandem expression vector for right and left mitoTALENs to cut kosena-type CMS gene orf125 (also cut Ogura CMS gene orf138)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK7WG2-mitoTAL1-orf125 was a gift from Shin-ichi Arimura (Addgene plasmid # 158736 ; http://n2t.net/addgene:158736 ; RRID:Addgene_158736)

  • For your References section:

    Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing. Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI. Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. Epub 2019 Jul 8. 10.1038/s41477-019-0459-z PubMed 31285556
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