pENTR E1 pF6A L1-L4
(Plasmid
#158729)
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PurposePlant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NG)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePINII
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Vector typeTALEN
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTALEN ORF without TAL repeat
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Multiste Gateway entry vector E1 compatible to be used as the final insertion vectors to make left TALEN, instead of ptCMV-136/63-VR-(NG), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR E1 pF6A L1-L4 was a gift from Shin-ichi Arimura (Addgene plasmid # 158729 ; http://n2t.net/addgene:158729 ; RRID:Addgene_158729) -
For your References section:
Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing. Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI. Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. Epub 2019 Jul 8. 10.1038/s41477-019-0459-z PubMed 31285556