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Addgene

pENTR E2 HSPter-35Sp-mito
(Plasmid #158727)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PINII
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A terminator of Arabidopsis heat shock protein, CAMV35S promoter, and mitochondrial presequence
  • Species
    Synthetic

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Multisite Gateway entry vector to terminate the E1 ORF and having CaMV35S promoter with mitochondrial presequence for the E3 ORF

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR E2 HSPter-35Sp-mito was a gift from Shin-ichi Arimura (Addgene plasmid # 158727 ; http://n2t.net/addgene:158727 ; RRID:Addgene_158727)

  • For your References section:

    Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing. Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI. Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. Epub 2019 Jul 8. 10.1038/s41477-019-0459-z PubMed 31285556
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