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Addgene

pDESTpK7WG2mito
(Plasmid #158726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7WG2
  • Backbone manufacturer
    VIB, Ghent Univ, Kamiri et al
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitochondrial presequence in front of attR1 in pk7WG2
  • Species
    Synthetic

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gateway destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the CaMV35S promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESTpK7WG2mito was a gift from Shin-ichi Arimura (Addgene plasmid # 158726 ; http://n2t.net/addgene:158726 ; RRID:Addgene_158726)

  • For your References section:

    Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing. Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI. Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. Epub 2019 Jul 8. 10.1038/s41477-019-0459-z PubMed 31285556
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