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Addgene

pYC1876
(Plasmid #158722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom design
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ssrA
  • Species
    Other
  • Insert Size (bp)
    39

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In Vivo Plasmid Editing Tool Based on CRISPR-Cas12a and Phage _ Red Recombineering

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYC1876 was a gift from Yi-Cheng Sun (Addgene plasmid # 158722 ; http://n2t.net/addgene:158722 ; RRID:Addgene_158722)

  • For your References section:

    A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC. mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19. 10.1128/mBio.02364-19 PubMed 31992616
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