pNHEJ-recAmu-sacB
(Plasmid
#158717)
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PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158717 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonecustom design
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerecA
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Alt namesacB
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SpeciesM. marinum
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MutationR61C
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: KpnI (destroyed), 3' Cloning Site: KpnI (destroyed)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNHEJ-recAmu-sacB was a gift from Yi-Cheng Sun (Addgene plasmid # 158717 ; http://n2t.net/addgene:158717 ; RRID:Addgene_158717) -
For your References section:
A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC. mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19. 10.1128/mBio.02364-19 PubMed 31992616
