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Addgene

pNHEJ-Cas12a
(Plasmid #158713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJV53-Cas12a
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
  • Species
    M. marinum
  • Insert Size (bp)
    4682

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: KpnI (not destroyed), 3' Cloning Site: NheI (destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNHEJ-Cas12a was a gift from Yi-Cheng Sun (Addgene plasmid # 158713 ; http://n2t.net/addgene:158713 ; RRID:Addgene_158713)

  • For your References section:

    A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC. mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19. 10.1128/mBio.02364-19 PubMed 31992616
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