TRE-mCherry-p2A-bReaches-TS
(Plasmid
#158701)
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PurposeReporter construct
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158701 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4365
- Total vector size (bp) 6165
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-p2A-bReaches-TS
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SpeciesSynthetic
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Insert Size (bp)1800
- Promoter TRE
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Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-minimal
- 3′ sequencing primer PolyA-r (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRE-mCherry-p2A-bReaches-TS was a gift from Alice Ting (Addgene plasmid # 158701 ; http://n2t.net/addgene:158701 ; RRID:Addgene_158701) -
For your References section:
Transcriptional readout of neuronal activity via an engineered Ca(2+)-activated protease. Sanchez MI, Nguyen QA, Wang W, Soltesz I, Ting AY. Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 10.1073/pnas.2006521117. 10.1073/pnas.2006521117 PubMed 33323488
