scFLARE1-p2A-eGFP
(Plasmid #158699)

• Purpose: Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
• Depositing Lab: Alice Ting
• Publication: Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 10.1073/pnas.2006521117.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3802
• Total vector size (bp): 8494
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: scFLARE1-p2A-eGFP
• Species: Synthetic
• Insert Size (bp): 4689
• Mutation: Truncated form of TEV protease (220-242 amino acids deleted, low affinity). S219V mutation improves stability, S153N mutation improves catalytic activity. Calmodulin from GCaMP5
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag, V5. eGFP

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-f
• 3′ sequencing primer: PolyA-r

Resource Information

• Supplemental Documents:
  • scFLARE1-p2a-eGFP.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry