Geminin-mStrawberry reporter
(Plasmid
#158690)
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PurposeThis plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLKO.1
- Backbone size w/o insert (bp) 7728
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemStrawberry
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SpeciesH. sapiens (human)
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Insert Size (bp)958
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byES-FUCCI
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Geminin-mStrawberry reporter was a gift from Ravid Straussman (Addgene plasmid # 158690 ; http://n2t.net/addgene:158690 ; RRID:Addgene_158690) -
For your References section:
Visual barcodes for clonal-multiplexing of live microscopy-based assays. Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, Straussman R. Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0. 10.1038/s41467-022-30008-0 PubMed 35585055
