AKT-translocation-mStrawberry reporter
(Plasmid #158685)

• Purpose: This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD
• Depositing Lab: Ravid Straussman
• Publication: Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.

Backbone

• Vector backbone: pLKO.1
• Backbone size w/o insert (bp): 7728
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mStrawberry
• Species: H. sapiens (human)
• Insert Size (bp): 3158
• Promoter: EF1a

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pLenti-FoxO1-Clover

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry