TRE reporter backbone
(Plasmid #158678)

• Purpose: (Empty Backbone) This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry
• Depositing Lab: Ravid Straussman
• Publication: Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.

Backbone

• Vector backbone: pLKO.1
• Backbone size (bp): 7728
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin
• Tag / Fusion Protein:
  • mStrawberry (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • mStrawberry (C terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mStrawberry-N1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry