mTAGBFP2 NES visual barcode
(Plasmid
#158667)
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Purposetagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WC
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLKO.1
- Backbone size w/o insert (bp) 7403
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTAGBFP2
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SpeciesH. sapiens (human)
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Insert Size (bp)786
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made bypBAD-mTagBFP2
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTAGBFP2 NES visual barcode was a gift from Ravid Straussman (Addgene plasmid # 158667 ; http://n2t.net/addgene:158667 ; RRID:Addgene_158667) -
For your References section:
Visual barcodes for clonal-multiplexing of live microscopy-based assays. Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, Straussman R. Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0. 10.1038/s41467-022-30008-0 PubMed 35585055
