pBsacA
(Plasmid #158650)

• Purpose: Constitutive transcription of sacA crRNA
• Depositing Lab: Wenjing Cui
• Publication: Cui Lab Plasmids (unpublished)

Backbone

• Vector backbone: pBSG03
• Backbone size w/o insert (bp): 6499
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology ; Shuttle vector Bacillus subtilis / E. coli
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Select for growth in Bacillus subtilis using Kanamycin.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sacA crRNA
• Alt name: homologous arm of sacA
• gRNA/shRNA sequence: AATTTCTACTGTTGTAGATTCGGCCGCCTCGATTATAACAAG
• Species: Francisella tularensis subsp. novicida
• Insert Size (bp): 1409
• Promoter: Pveg

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AAATACTACCTGTCCCTTGCT

Resource Information

• Supplemental Documents:
  • pBsacA.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry