pSLIK 3xFLAG-MAVS-Gln93 hygro
(Plasmid
#158635)
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PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSLIK hygro
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Backbone manufacturerAddgene #25737
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMAVS
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Alt nameIPS-1
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Alt nameVISA
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SpeciesH. sapiens (human)
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MutationE93Q
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Entrez GeneMAVS (a.k.a. CARDIF, IPS-1, IPS1, VISA)
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Tag
/ Fusion Protein
- 3XFLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). For pSLIK vectors, it is recommended to use zeocin in addition to ampicillin for bacterial selection.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLIK 3xFLAG-MAVS-Gln93 hygro was a gift from Kevin Janes (Addgene plasmid # 158635 ; http://n2t.net/addgene:158635 ; RRID:Addgene_158635) -
For your References section:
Modeling the complete kinetics of coxsackievirus B3 reveals human determinants of host-cell feedback. Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, Shah M, Janes KA. Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 10.1016/j.cels.2021.02.004. Epub 2021 Mar 18. 10.1016/j.cels.2021.02.004 PubMed 33740397