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Addgene

pet11a-ABLE
(Plasmid #158627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet11a
  • Backbone manufacturer
    genscript
  • Backbone size w/o insert (bp) 5641
  • Total vector size (bp) 6061
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Apixaban-Binding Helical Bundle
  • Alt name
    ABLE
  • Species
    Synthetic
  • Insert Size (bp)
    420
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His-tag and TEV cleavage tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet11a-ABLE was a gift from William DeGrado (Addgene plasmid # 158627 ; http://n2t.net/addgene:158627 ; RRID:Addgene_158627)

  • For your References section:

    A defined structural unit enables de novo design of small-molecule-binding proteins. Polizzi NF, DeGrado WF. Science. 2020 Sep 4;369(6508):1227-1233. doi: 10.1126/science.abb8330. 10.1126/science.abb8330 PubMed 32883865
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