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Addgene

MX01
(Plasmid #158624)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 158624 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MX01
  • Backbone manufacturer
    Shubham Singh
  • Backbone size (bp) 8755
  • Modifications to backbone
    New backbone, synthesized by using Gibson Assembly.
  • Vector type
    Mammalian Expression ; GATEWAY Destination Vector ; BacMam Acceptor Vector
  • Promoter CMV-IE
  • Tag / Fusion Protein
    • P2A (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Gentamicin, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MX01 was a gift from Timm Maier (Addgene plasmid # 158624 ; http://n2t.net/addgene:158624 ; RRID:Addgene_158624)

  • For your References section:

    The 3.2-A resolution structure of human mTORC2. Scaiola A, Mangia F, Imseng S, Boehringer D, Berneiser K, Shimobayashi M, Stuttfeld E, Hall MN, Ban N, Maier T. Sci Adv. 2020 Nov 6;6(45). pii: 6/45/eabc1251. doi: 10.1126/sciadv.abc1251. Print 2020 Nov. 10.1126/sciadv.abc1251 PubMed 33158864
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