TwinStrep-SUMO-NLS-BhCas12b_v4
(Plasmid
#158615)
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PurposeFor protein expression of BhCas12b_v4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTwinStrep-SUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNLS-BhCas12b_v4
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Tag
/ Fusion Protein
- 6xHIS-TwinStrep-SUMO (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TwinStrep-SUMO-NLS-BhCas12b_v4 was a gift from Feng Zhang (Addgene plasmid # 158615 ; http://n2t.net/addgene:158615 ; RRID:Addgene_158615) -
For your References section:
Engineering of CRISPR-Cas12b for human genome editing. Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F. Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4. 10.1038/s41467-018-08224-4 PubMed 30670702