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Addgene

pCASCADE-gltA2-xylA
(Plasmid #158613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCASCADE
  • Backbone manufacturer
    None - Constructed in House
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 2904
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gltA2-xylA guide array
  • Species
    Synthetic
  • Promoter E . coli ugpB gene promoter, low phosphate induction

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGGAGACCACAACGG
  • 3′ sequencing primer CGCAGTCGAACGACCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Read the preprint at bioRxiv: https://doi.org/10.1101/2020.07.27.222588

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCASCADE-gltA2-xylA was a gift from Michael Lynch (Addgene plasmid # 158613 ; http://n2t.net/addgene:158613 ; RRID:Addgene_158613)

  • For your References section:

    Dynamic control over feedback regulatory mechanisms improves NADPH flux and xylitol biosynthesis in engineered E. coli. Li S, Ye Z, Moreb EA, Hennigan JN, Castellanos DB, Yang T, Lynch MD. Metab Eng. 2021 Jan 15. pii: S1096-7176(21)00013-6. doi: 10.1016/j.ymben.2021.01.005. 10.1016/j.ymben.2021.01.005 PubMed 33460820
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