V2rf2-IRES-BL Targeting Vector
(Plasmid #15860)

• Depositing Lab: Peter Mombaerts
• Publication: Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Backbone

• Vector backbone: pBS-SK+ with modification of PmeI and AscI into KpnI
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: V2rf2
• Alt name: mV2R58
• Alt name: EC1-V2R
• Alt name: Vmn2r81
• Species: M. musculus (mouse)
• Insert Size (bp): 13505
• Entrez Gene: Vmn2r81 (a.k.a. V2rf2, EC1-V2R, EC1-VR2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry