M10.2::GFP-ACNF Targeting Vector
(Plasmid
#15859)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS-SK+ with modification of PmeI and AscI into KpnI
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM10.2
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SpeciesM. musculus (mouse)
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Insert Size (bp)13036
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M10.2::GFP-ACNF Targeting Vector was a gift from Peter Mombaerts (Addgene plasmid # 15859 ; http://n2t.net/addgene:15859 ; RRID:Addgene_15859) -
For your References section:
Expression of nonclassical class I major histocompatibility genes defines a tripartite organization of the mouse vomeronasal system. Ishii T, Mombaerts P. J Neurosci. 2008 Mar 5. 28(10):2332-41. 10.1523/JNEUROSCI.4807-07.2008 PubMed 18322080