Sticky-147-4X
(Plasmid
#158575)
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Purpose(Empty Backbone) Production of DNA fragment for nucleosome core particle preparation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Backbone manufacturerGenscript
- Backbone size (bp) 2710
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Modifications to backbonecloned into the HindIII and EcoRI sites of the pUC19 vector
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Vector typeBacterial vector for DNA production
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please cite the following article in any publications where this construct is utilized:
Sharma D, De Falco L, Padavattan S, Rao C, Geifman-Shochat S, Liu CF, Davey CA. 2019. PARP1 exhibits enhanced association and catalytic efficiency with gammaH2A.X-nucleosome. Nat. Commun.10(1): 5751. doi: 10.1038/s41467-019-13641-0.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sticky-147-4X was a gift from Curt Davey (Addgene plasmid # 158575 ; http://n2t.net/addgene:158575 ; RRID:Addgene_158575) -
For your References section:
PARP1 exhibits enhanced association and catalytic efficiency with gammaH2A.X-nucleosome. Sharma D, De Falco L, Padavattan S, Rao C, Geifman-Shochat S, Liu CF, Davey CA. Nat Commun. 2019 Dec 17;10(1):5751. doi: 10.1038/s41467-019-13641-0. 10.1038/s41467-019-13641-0 PubMed 31848352
