601L-145-8X
(Plasmid
#158572)
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Purpose(Empty Backbone) Production of DNA fragment for nucleosome core particle preparation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Backbone manufacturerEZbiolab
- Backbone size (bp) 2710
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Modifications to backboneInsertion at EcoRV site
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Vector typeBacterial vector for DNA production
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please cite the following article in any publications where this construct is utilized:
E.Y.D. Chua, D. Vasudevan, G.E. Davey, B. Wu & C.A. Davey. 2012. The Mechanics Behind DNA Sequence-Dependent Properties of the Nucleosome. Nucleic Acids Res. 40(13): 6338-6352.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
601L-145-8X was a gift from Curt Davey (Addgene plasmid # 158572 ; http://n2t.net/addgene:158572 ; RRID:Addgene_158572) -
For your References section:
The mechanics behind DNA sequence-dependent properties of the nucleosome. Chua EY, Vasudevan D, Davey GE, Wu B, Davey CA. Nucleic Acids Res. 2012 Jul;40(13):6338-52. doi: 10.1093/nar/gks261. Epub 2012 Mar 26. 10.1093/nar/gks261 PubMed 22453276