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Addgene

pLIX403-EBFP2-ccdB-EYFP
(Plasmid #158558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIX403
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    EYFP
  • Tag / Fusion Protein
    • EBFP2 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: PacI (not destroyed), 3' Cloning Site: SbfI (not destroyed)

Please note: Plasmid contains an IS1 insertion. This insertion is not expected to affect plasmid function as the sequence is not in a coding region of the plasmid and is outside of the lentiviral LTRs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIX403-EBFP2-ccdB-EYFP was a gift from Alejandro Chavez (Addgene plasmid # 158558 ; http://n2t.net/addgene:158558 ; RRID:Addgene_158558)
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