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Addgene

pGEX-HiGlpG- S116A, H196A
(Plasmid #158539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PGEX-6P-1
  • Backbone size w/o insert (bp) 4953
  • Total vector size (bp) 5532
  • Modifications to backbone
    -
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HiGlpG
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
    579
  • Mutation
    S116A;H169A
  • GenBank ID
    NC_000907.1
  • Tag / Fusion Protein
    • GST tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site - (destroyed during cloning)
  • 5′ sequencing primer PGEX-5'
  • 3′ sequencing primer PGEX-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-HiGlpG- S116A, H196A was a gift from Sinisa Urban (Addgene plasmid # 158539 ; http://n2t.net/addgene:158539 ; RRID:Addgene_158539)

  • For your References section:

    Decoding the Functional Evolution of an Intramembrane Protease Superfamily by Statistical Coupling Analysis. Mihaljevic L, Urban S. Structure. 2020 Aug 10. pii: S0969-2126(20)30279-3. doi: 10.1016/j.str.2020.07.015. 10.1016/j.str.2020.07.015 PubMed 32795403
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