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Addgene

pMXs-Pou4f3
(Plasmid #158530)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158530 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMXs-GW
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Since viral vectors are prone to recombination, we recommend testing 2-4 colonies to help select the full plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POU Class 4 Homeobox 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1017
  • Entrez Gene
    POU4F3 (a.k.a. BRN3C, DFNA15, DFNA42, DFNA52)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-Pou4f3 was a gift from Neil Segil (Addgene plasmid # 158530 ; http://n2t.net/addgene:158530 ; RRID:Addgene_158530)
  • For your References section:

    Generation of inner ear hair cells by direct lineage conversion of primary somatic cells. Menendez L, Trecek T, Gopalakrishnan S, Tao L, Markowitz AL, Yu HV, Wang X, Llamas J, Huang C, Lee J, Kalluri R, Ichida J, Segil N. Elife. 2020 Jun 30;9. pii: 55249. doi: 10.7554/eLife.55249. 10.7554/eLife.55249 PubMed 32602462